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Aline Muro, Undergraduate Student, Biology Major
Martina Perez, Undergraduate Student, Biology Major
My group is determining if rotenone, a chemical known to induce reactive oxygen species (ROS) and interfere with the oxidative status of cells, causes VL3-3M2 cells oxidative status to be affected. To do so, we performed an EC50 assay to determine what concentration of rotenone would cause 50% of the cells to die, therefore implying it is a good positive control. If rotenone is a good positive control, we can utilize it to see how ROS affect VL3-3M2 cells, which are being used as a model for embryonic thymocytes, in the presence of an endocrine disrupting chemical using the H2DCFDA staining assay.